MAK447

Alkaline Phosphatase Assay Kit

Sufficient for 250 colorimetric tests

• Synonym(s): ALP Activity Assay Kit
• UNSPSC Code: 12161503

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: colorimetric
• relevant disease(s): osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer
• storage temp.: −20°C

Description

General description

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

Application

The Alkalnine Phosphatase Assay Kit may be used for:

• Cancer Research
• Gastroenterology Disease Research
• Osteoporosis Research

Features and Benefits

Enhanced Detection Range: Detect ALP levels accurately across a wide range, from 2 U/L to 800 U/L, using only 5 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.

Principle

The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction isdirectly proportional to the enzyme activity.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Pictograms: GHS05,GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H290 - H302 + H332 - H314 - H341 - H373 - H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3