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MAK001

Sigma-Aldrich

Alanine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

Alanine is a nonessential amino acid that plays a key role in the glucose-alanine cycle between muscle tissue and the liver. In amino acid-degrading tissues such as muscle, amino groups are pooled as glutamate by transamination reactions. The amino group of glutamate is transferred to pyruvate via alanine aminotransferase, forming alanine and α-ketoglutarate. The alanine is passed into the blood and transported to the liver. This reaction is reversed in the liver where pyruvate can be used in gluconeogenesis to form glucose, which may return to other tissues through the circulatory system. Increased alanine levels correlate with higher blood pressure, energy intake, cholesterol levels, and body mass index.

Application

Alanine Assay Kit has been used to determine the intracellular alanine pool.

Suitability

Suitable for alanine detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

In this kit, alanine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the alanine present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

replaced by

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Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

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Mauro D P.
Amino Acids and Proteins for the Athlete: The Anabolic Edge (1997)
The complex role of branched chain amino acids in diabetes and cancer.
O'Connell T M.
Metabolites, 3(4), 931-945 (2013)
GABA transaminases from Saccharomyces cerevisiae and Arabidopsis thaliana complement function in cytosol and mitochondria.
Cao J, et al.
Yeast, 30(7), 279-289 (2013)
Berg J M, et al.
Biochemistry (5th Edition) (2002)
Dmitry Frank et al.
Neuropharmacology, 155, 173-184 (2019-06-04)
Post-stroke depression (PSD) is a common and serious complication following stroke. Both stroke and depression have independently been associated with pathologically elevated glutamate levels in the brain's extra-cerebral fluid (ECF). Here we evaluate an alternative therapeutic approach to PSD with
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Protocols

Enzymatic Method for Determining Alanine (Alanine Assay)

Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.

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