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Key Documents

M6899

Sigma-Aldrich

Murashige and Skoog Basal Salts with minimal organics

powder, suitable for plant cell culture

Synonym(s):

MSMO

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

With the macro- and micronutrients, and vitamins as described by Linsmaier and Skoog (1965).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M6899 is a variant media) with macro- and micronutrients, vitamins as described by Linsmaier and Skoog.

Pictograms

Flame over circleExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 2

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Organic growth factor requirements of tobacco tissue cultures.
Linsmaier, E.M. and Skoog, F.
Physiologia Plantarum, 18, 100-127 (1965)
Qiaojun Jia et al.
BMC genomics, 16, 927-927 (2015-11-18)
sdw1/denso is one of the most important and useful semi-dwarf genes in barley breeding. At least four sdw1/denso alleles have been reported and HvGA20ox 2 is considered as the candidate gene. Up to date, results of studies have not univocally
Li Wang et al.
Planta, 249(4), 1107-1118 (2018-12-16)
In hybrids of Arabidopsis, cotyledons influence the amount and proportion of hybrid vigor in total plant growth. We found Arabidopsis cotyledons are essential for plant growth and in some hybrids for hybrid vigor. In hybrids between C24 and Landsberg erecta
Okhee Choi et al.
Plant physiology, 146(2), 657-668 (2007-12-07)
Pseudomonas fluorescens B16 is a plant growth-promoting rhizobacterium. To determine the factors involved in plant growth promotion by this organism, we mutagenized wild-type strain B16 using OmegaKm elements and isolated one mutant, K818, which is defective in plant growth promotion
Thelma F Madzima et al.
Frontiers in plant science, 12, 694289-694289 (2021-09-08)
Plants respond to abiotic stress stimuli, such as water deprivation, through a hierarchical cascade that includes detection and signaling to mediate transcriptional and physiological changes. The phytohormone abscisic acid (ABA) is well-characterized for its regulatory role in these processes in

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