M4782

Mutanolysin from Streptomyces globisporus ATCC 21553

0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)

• CAS Number: 55466-22-3
• MDL number: MFCD00131635
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• Quality Level: 200
• sterility: 0.2 μm filtered
• form: lyophilized powder
• specific activity: ≥4000 units/mg protein (biuret)
• mol wt: 23 kDa
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Unit Definition

One unit will produce a ΔA₆₀₀ of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll and sodium succinate buffer salts

Preparation Note

Prepared from M 9901

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves