type
Type IX
form
beads
technique(s)
liquid chromatography (LC): suitable
storage temp.
2-8°C
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Application
Hydroxyalkoxypropyl-Dextran is used for liquid chromatography, protein chromatography, and gel filtration chromatography. Hydroxyalkoxypropyl-Dextran has been used to develop a nondestructive method for effective removal of lipids from small quantities of aqueous protein as well as to develop a highly specific method for the determination of cortisol in human serum and urine.
Lipophilic, hydrophobic gel for liquid chromatography.
Other Notes
Hydroxypropyl beaded dextran (Lipophilic Sephadex, LH-20-100), substituted with long chain alkyl ethers.
Substituted approx. 50% by weight with alkyl chains of length C15-C18
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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U Kragh-Hansen
Analytical biochemistry, 210(2), 318-327 (1993-05-01)
A simple and nondestructive method was developed for effective removal of lipids, such as long-chain fatty acids and steroids, from small quantities (2-10 mg) of aqueous protein. The procedure operates with a high recovery of protein (97%) and was elaborated
J B Lowe et al.
The Journal of biological chemistry, 262(12), 5931-5937 (1987-04-25)
Rat intestinal fatty acid-binding protein (I-FABP) is an abundant, 15,124-Da polypeptide found in the cytosol of small intestinal epithelial cells (enterocytes). It is homologous to rat liver fatty acid-binding protein (L-FABP), a 14,273-Da cytosolic protein which is found in enterocytes
D Apter et al.
Clinica chimica acta; international journal of clinical chemistry, 63(2), 139-148 (1975-09-01)
A highly specific method for the determination of cortisol in human serum and urine is described. The sample is first extracted with diethyl ether/ethyl acetate (1 : 1, by vol.), then chromatographed on a highly lipophilic derivative of Sephadex (hydroxyalkoxypropyl
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