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GE17-5318-06

Ni Sepharose™ 6 Fast Flow

Cytiva 17-5318-06, pack of 5 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-5318-06

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

~40 mg binding capacity(histidine-tagged protein)

suitability

suitable for bioprocess medium

General description

Ni Sepharose™ 6 Fast FLow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose™ Fast FLow platform.

Application

Ni Sepharose™ 6 Fast FLow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged Proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.

Ni Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Ni Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Designed for scaling up purification of histidine-tagged Proteins
  • High Protein binding capacity and minimal leakage of Ni2? ions
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep™ and HisTrap™ column formats.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pär Comstedt et al.
PloS one, 9(11), e113294-e113294 (2014-11-20)
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