Skip to Content
Merck
All Photos(1)

Documents

GE17-5123-10

benzamidin Seph 4Fast Flow H-sub

Cytiva 17-5123-10, pack of 25 mL

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

p-aminobenzamidine

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-5123-10

storage condition

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

matrix

highly cross-linked 4% agarose

average diameter

90 μm

cleaning in place

1-9

working range

2-8

storage temp.

2-8°C

Related Categories

General description

HiTrap® Benzamidine FF (high sub) is an excellent tool for removal of proteolytic activity from a protein.

Application

Benzamidine Sepharose™ 4 Fast FLow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates

Features and Benefits

  • Benzamidine Sepharose™ 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
  • p-aminobenzamidine (pABA) immobilized on Sepharose™ 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
  • For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens

This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

Principles and standard conditions for different purification techniques

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from GE Healthcare.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

See All

Protocols

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service