Hep2 (HeLa derivative) Cell Line human
86030501, human cervix, Epithelial
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About This Item
UNSPSC Code:
41106514
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product name
Hep2 (HeLa derivative) Cell Line human, 86030501
biological source
human cervix
description
Human Black, cervix carcinoma
growth mode
Adherent
karyotype
Modal no. 14
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human cervix carcinoma
Cell Line Description
Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Application
Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. After thawing the first subculture may take 7-10 days.
Other Notes
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Certificates of Analysis (COA)
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