B9754

BIS-TRIS

≥98.0% (titration)

• Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
• Empirical Formula (Hill Notation): C₈H₁₉NO₅
• CAS Number: 6976-37-0
• Molecular Weight: 209.24
• Beilstein: 2205275
• EC Number: 230-237-7
• MDL number: MFCD00002853
• UNSPSC Code: 12161700
• PubChem Substance ID: 24892155
• NACRES: NA.25

Properties

• Assay: ≥98.0% (titration)
• form: crystalline powder
• storage condition: dry at room temperature
• technique(s): UV/Vis spectroscopy: suitable; electrophoresis: suitable
• color: white
• useful pH range: 5.8-7.2
• pKa (25 °C): 6.5
• solubility: H₂O: 500 mg/mL, clear, colorless to very faintly yellow
• suitability: suitable for Western blot; suitable for column chromatography of proteins
• application(s): diagnostic assay manufacturing; general analytical; microbiology
• SMILES string: OCCN(CCO)C(CO)(CO)CO
• InChI: 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• InChI key: OWMVSZAMULFTJU-UHFFFAOYSA-N

Description

General description

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

Application

Bis-Tris has been used :

• as a buffer for Electrophoresis
• as a component of transfer buffer for Western Blotting

Features and Benefits

• Suitable for Biological and Biochemical Research
• Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves