B2680

Bone Morphogenetic Protein 4 human

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonym(s): BMP-4
• MDL number: MFCD01867024
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in NSO cells
• Assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 10-30 ng/mL ED₅₀
• mol wt: monomer calculated mol wt 13 kDa; ~22 kDa by SDS-PAGE
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/mg Endotoxin level (LAL test)
• UniProt accession no.: P12644
• storage temp.: −20°C
• Gene Information: human ... BMP4(652)

Description

Biochem/physiol Actions

Cellular responses to BMP-4 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. BMPs stimulate angiogenesis by inducing the production of VEGF A by osteoblasts. Mature human and mouse BMP-4 demonstrates 98% and 100% homology, respectively, to mature rat BMP-4.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to induce alkaline phosphatase production by C2C12 myoblasts.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable