Skip to Content
Merck
All Photos(1)

Key Documents

A9776

Sigma-Aldrich

α-Actinin from chicken gizzard

~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
11003-00-2
MDL number:
MFCD00130473
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

chicken gizzard

Assay

~80% α-actinin basis (SDS-PAGE)

form

ammonium sulfate suspension

mol wt

100 kDa

UniProt accession no.

P05094
P12814
P20111
Q7TPR4
Q90734
Q9Z1P2

storage temp.

2-8°C

Gene Information

chicken ... ACTN2(396263) , ACTN4(396024) , RCJMB04_23c5(373918)
human ... ACTN1(87)
mouse ... ACTN1(109711)
rat ... ACTN1(81634)

General description

α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.

Application

α-Actinin from chicken gizzard has been used:
  • as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones
  • in in vitro motility experiments to test its effect on actin filament movement
  • to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies

Biochem/physiol Actions

α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.

Packaging

Package size based on protein content

Physical form

Suspension in 2 M (NH4)2SO4 containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride

Preparation Note

Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.

Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Anja Katzemich et al.
PLoS genetics, 9(3), e1003342-e1003342 (2013-03-19)
The Drosophila Alp/Enigma family protein Zasp52 localizes to myotendinous junctions and Z-discs. It is required for terminal muscle differentiation and muscle attachment. Its vertebrate ortholog ZASP/Cypher also localizes to Z-discs, interacts with α-actinin through its PDZ domain, and is involved
F Rivero et al.
Journal of cell science, 112 ( Pt 16), 2737-2751 (1999-07-22)
The contribution of three actin cross-linking proteins, alpha-actinin (alphaA), gelation factor (ABP-120), and the 34 kDa actin-bundling protein to cellular functions has been studied in three single mutant (alphaA-, 120-, and 34-) and three double mutant (alphaA-/120-, 34-/alphaA-, 34-/120-) strains
Wiebke Onkes et al.
Genes, chromosomes & cancer, 52(5), 512-522 (2013-01-31)
About 20% of ovarian carcinomas show alterations of 19p13 and/or 19q13 in the form of added extra material whose origin often is from chromosome 11. Based on earlier spectral karyotype analysis of the ovarian cancer cell line SKOV-3, which shows
Nicola Cacciani et al.
Acta physiologica (Oxford, England), 229(1), e13425-e13425 (2019-12-05)
Critical illness myopathy (CIM) represents a common consequence of modern intensive care, negatively impacting patient health and significantly increasing health care costs; however, there is no treatment available apart from symptomatic and supportive interventions. The chaperone co-inducer BGP-15 has previously
Meishan Li et al.
The Journal of physiology, 588(Pt 24), 5105-5114 (2010-10-27)
Muscle, motor unit and muscle fibre type-specific differences in force-generating capacity have been investigated for many years, but there is still no consensus regarding specific differences between slow- and fast-twitch muscles, motor units or muscle fibres. This is probably related
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service