Skip to Content
Merck
All Photos(1)

Key Documents

More Documents

73737

Sigma-Aldrich

4-Nitrophenyl phosphate bis(tris) salt

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

Synonym(s):

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
68189-42-4
Molecular Weight:
461.36
Beilstein:
1578142
EC Number:
269-198-6
MDL number:
MFCD00037009
UNSPSC Code:
12352204
PubChem Substance ID:
57652488
NACRES:
NA.32

product name

4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, ≥97.0% (enzymatic)

Assay

≥97.0% (enzymatic)

form

powder or crystals

quality

for the determination of phosphatase

impurities

≤0.1% free nitrophenol
≤6% water

solubility

H2O: 0.1 g/mL, clear

storage temp.

2-8°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.

Biochem/physiol Actions

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Elif Özlem Arslan-Aydoğdu et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(1), 189-199 (2017-10-19)
Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase
D.W. Moss
Methods of Enzymatic Analysis, 92-92 null
Aneta Pietraszek et al.
Colloids and surfaces. B, Biointerfaces, 173, 1-8 (2018-09-28)
Alkaline phosphatase (ALP) was sucessfully incorporated into the halloysite (HAL) nanotubes, as confirmed by FTIR-ATR and XRD analyses. The loading efficiency (LE) of ALP was found to be 13.5%, while its encapsulation efficiency (EE) was estimated to be around 27%
O.H. Lowry
Methods in Enzymology, 4, 366-366 (1957)
Mary E Wood et al.
Vaccine, 36(50), 7737-7743 (2018-11-12)
Chronic wasting disease (CWD) is a fatal prion disease affecting multiple cervid species. Effective management tools for this disease, particularly in free-ranging populations, are currently limited. We evaluated a novel CWD vaccine in elk (Cervus canadensis) naturally exposed to CWD
View All Related Papers

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service