B6529

Brilliant Blue R Staining Solution

suitable for (for immunoelectrophoresis protein staining)

• Synonym(s): Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
• Empirical Formula (Hill Notation): C₄₅H₄₄N₃NaO₇S₂
• CAS Number: 6104-59-2
• Molecular Weight: 825.97
• Colour Index Number: 42660
• Beilstein: 5718025
• MDL number: MFCD00041762
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891914
• NACRES: NA.47

Properties

• form: liquid
• Quality Level: 200
• color: dark blue
• suitability: suitable for (for immunoelectrophoresis protein staining)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
• InChI key: NKLPQNGYXWVELD-UHFFFAOYSA-M

Description

Application

Brilliant Blue R staining solution is especially designed for use in staining protein in agarose gels following immunoelectrophoresis and Ouchterlony gels. The stain contains ethanol and acetic acid so gels do not require fixing prior to staining. The immunoelectrophoresis or Ouchterlony gel is first washed with water and saline to remove non-precipitated proteins and then dried. The gel is then immersed in staining solution for 30 min and destained with 10% acetic acid.

For detection of protein bands following electrophoresis.

Components

0.5% (w/v) Brilliant Blue R, 45% (v/v) ethanol and 10% (v/v) acetic acid.

Analysis Note

Tested for suitability on agarose gels following immunoelectrophoresis.

Legal Information

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Warning
• Hazard Statements: H226 - H315 - H319
• Precautionary Statements: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 81.0 °F - closed cup
• Flash Point(C): 27.2 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves