213519

Pyronin Y

certified by the Biological Stain Commission, Dye content 50 %

• Synonym(s): Pyronin G, Pyronin J
• Empirical Formula (Hill Notation): C₁₇H₁₉ClN₂O
• CAS Number: 92-32-0
• Molecular Weight: 302.80
• Colour Index Number: 45005
• Beilstein: 3795789
• EC Number: 202-147-8
• MDL number: MFCD00011725
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852787
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder or crystals
• composition: Dye content, 50%
• mp: 250-260 °C (lit.)
• λmax: 548 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
• InChI: 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
• InChI key: INCIMLINXXICKS-UHFFFAOYSA-M

Description

General description

Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.

Application

Pyronin Y is commonly used in histology as a constituent of the ethyl (methyl) green-pyronine method for demonstrating DNA and RNA. It is known as a nucleic acid stain for both RNA and DNA. Additionally, it is suitable for other microscopic applications including demonstration of lipofuscin in neurons and simultaneous fixation and staining of phospholipids.

Suitability

Certified for use in the methyl green-pyronin method for differential staining of nucleic acids.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves