11202375001
Roche
DOTAP Liposomal Transfection Reagent
>99% (TLC), liquid, suitable for transfection
Synonym(s):
DOTAP methosulfate, liposomal transfection reagent, dotap, transfection reagent, dotap, N-(2,3-Dioleoyloxy-1-propyl)trimethylammonium methyl sulfate, Transfection reagent
About This Item
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description
N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate
Quality Level
Assay
>99% (TLC)
form
liquid
packaging
pkg of 5 × 400 μL (10 U/μl)
manufacturer/tradename
Roche
technique(s)
transfection: suitable
storage temp.
2-8°C
SMILES string
[H]C(C[N+](C)(C)C)(OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O.O=S([O-])(OC)=O
InChI
1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1/b22-20-,23-21-;
InChI key
RSMRWWHFJMENJH-LQDDAWAPSA-M
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General description
Application
Features and Benefits
- Easy to use: The lipid dispersion is simply mixed with the DNA solution, then applied directly to the cells
- Highly effective: 5 to 100-fold more effective than the calcium phosphate or DEAE-dextran method
- Gentle: No cytotoxic effects
- Flexible: Equally effective in the presence or absence of serum. Effective on a wide range of species (e.g., insect cells, mammalian cells) and a variety of different cell types. Can be used for either transient or stable transfection procedures.
- Aqueous dispersion (liposomes) in MBS (MES-buffered saline, pH 6.2) (bottled under argon), 1mg/ml, sterile-filtered.
Preparation Note
The optimal working concentration is dependent on several parameters, including the cell line being used, concentration and type of nucleic acid (DNA, RNA), and incubation time. It is important to optimize the transfection conditions for the individual cell type studied.
Cytotoxicity: Not cytotoxic up to a concentration of 100μg/ml (PBLs, HeLa cells).
Other Notes
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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