10108987001
Roche
Pyrophosphatase, inorganic (PPase)
from yeast
Synonym(s):
inorganic pyrophosphatase
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About This Item
biological source
yeast
Quality Level
form
suspension
specific activity
~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)
packaging
pkg of 1 mL (1 mg)
manufacturer/tradename
Roche
storage temp.
2-8°C
General description
Pyrophosphate phosphohydrolase
The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.
Specificity
Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.
Application
Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.
Pyrophosphatase, inorganic (PPase) has been used in :
- Enzymatic nitric oxide (NO) detection system
- In vitro transcription
- Multiply-primed rolling circle amplification
Biochem/physiol Actions
Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.
Quality
Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
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