SCM087
Mouse iPS Reprogramming Boost Supplement
The Mouse iPS Reprogramming Boost Supplement enhances the efficiency of mouse iPS colony formation by at least 3-fold.
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General description
The Mouse iPS Reprogramming Boost Supplement contains three proprietary small molecules in amounts sufficient to supplement over 250 mL mouse ES/iPS cell maintenance medium. When used in conjunction with the Mouse STEMCCA lentivirus reprogramming kits (SCR530, SCR531), the Mouse iPS Reprogramming Boost Supplement enhanced the efficiency of mouse iPS colony formation by at least 3-fold. Mouse iPS Reprogramming Boost Supplement can be used to enhance reprogramming efficiency in somatic cell lines that may be more refractory to reprogramming. Mouse iPS cells generated through use of the Mouse iPS Reprogramming Boost Supplement display characteristic ES cell-like morphology, stained positive for alkaline phosphatase, expressed the correct mouse ES cell marker phenotype (SSEA-1 and Sox-2) and can be rapidly expanded in normal mouse ES cell culture conditions.
Components
1. TGF-ß RI Kinase Inhibitor II Supplement (1000X): (Part No. CS204458) One (1) vial containing 300 µL of the inhibitor in high quality DMSO.
2. GSK3ß Inhibitor Supplement (1000X): (Part No. CS204418) One (1) vial containing 300 µL of the inhibitor in high quality DMSO.
3. Methylation Modulator-A Supplement (1000X): (Part No. CS204419) One (1) vial containing 300 µL of the inhibitor in water.
Storage and Stability
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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