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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
Y12, monoclonal
mol wt
calculated mol wt 25 kDa
observed mol wt ~25 kDa
purified by
using protein G
species reactivity
human, mouse
packaging
antibody small pack of 100 μL
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Smith Antigen in HCT116 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Smith Antigen in Caco-2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected Smith Antigen in Immunocytochemistry (dos Santos, N.R. et al. (1997). Hum Mol Genet. 6(9): 1549-58).
Western Blotting Analysis: A representative lot detected Smith Antigen in Western Blotting applications (Naro, C. et al. (2019). Cell Rep. 26(11): 2929-2941; Somarelli, J.A., et al. (2011). Lupus. 20(3): 274-89; Brahms, H. et al. (2000). J Biol Chem. 275(22): 17122-9).
RNA Binding Protein Immunoprecipitation (RIP): A representative lot detected Smith Antigen in RNA Binding Protein Immunoprecipitation applications (Naro, C. et al. (2019). Cell Rep. 26(11):2929-2941).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Smith Antigen in human tonsil tissue sections.
ELISA Analysis: A representative lot detected Smith Antigen in ELISA applications (Zhou, Y, et al. (2017). J Immunol. 198(5):1846-1854).
Immunohistochemistry Applications: A representative lot detected Smith Antigen in Immunohistochemistry applications (Mirra, A., et al. (2017). Sci Rep. 7(1):2033).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Smith Antigen in Immunoprecipitation applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA. 78(5):2737-41; Naro, C. et al. (2019). Cell Rep. 26(11): 2929-2941; Mirra, A., et al. (2017). Sci Rep.;7(1): 2033; Fischer, U. et al. (1997). Cell. 90(6):1023-9).
Radioimmunoassay: A representative lot detected Smith Antigen in Radioimmunoassay applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA.;78(5):2737-41).
Immunofluorescence Analysis: A representative lot detected Smith Antigen in Immunofluorescence applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA.78(5):2737-41; Mirra, A., et al. (2017). Sci Rep. 7(1):2033).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Storage and Stability
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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