General description
A peptide corresponding to human Histone H3.3.
Specificity
This antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.
Application
Multiplex Analysis (Mplex): 0.05-0.5 μg/mL from a representative lot was used in Multiplex.
Quality
Evaluated by Western Blotting in HeLa whole cell lysate, recombinant Histone H3.3 and recombinant Histone H3.1.
Western Blotting Analysis (WB): 1 μg/mL of this antibody detected Histone H3.3 in HeLa whole cell lysate and recombinant Histone H3.3.
Western Blotting Analysis (WB): 1 μg/mL of this antibody detected Histone H3.3 in HeLa whole cell lysate and recombinant Histone H3.3.
Physical properties
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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