MAB88916-C
Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free
clone 3E3, from mouse
Synonym(s):
Fibronectin, FN, CIG, Cold-insoluble globulin
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3E3, monoclonal
species reactivity
human
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FN1(2335)
General description
Fibronectin (FN), or in earlier literature also known as Cold-insoluble globulin (CIG), and is encoded by the gene named FN1/FN is an extracellular matrix protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Fibronectin also plays an important role in bone development and bone structure formation. Additionally, when fibronectin binds the protein anastellin it forms a multiprotein complex termed superfibronectin that has been shown to inhibit tumor growth, angiogenesis and metastasis. Fibronectin protein can be found extracellularly as well as intracellularly. There are multiple isoforms produced by alternative splicing. Plasma fibronectin (a soluble dimeric form) is secreted by hepatocytes into the blood. Cellular fibronectin (dimeric or cross-linked multimeric forms), are made by fibroblasts, epithelial and other cell types, and is deposited as fibrils in the extracellular matrix.
Immunogen
Epitope: This antibody recognizes the cell attachment fragment of Fibronectin.
Purified human Fibronectin, which contains the Cell Attachment Fragment, from amnion liquid.
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free is validated for use in western blotting, IHC, ELISA, ICC & inhibition for the detection of Fibronectin.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of U-251 cell lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Fibronectin (Cell Attachment Fragment) in human placenta and liver tissues.
ELISA Analysis: A representative lot detected the conformation of Fibronectin adsorbed onto self-assembled monolayers (SAMs) of alkanethiols over a range of Fibroectin surface densities (Kesolowsky, B. G., et al. (2003). J Biomed Mater Res A. 66(2):247-259.).
Immuncytochemistry Analysis: A representative lot detected Fibronectin (Cell Attachment Fragment) in Extracellular matrix deposited by HUVECs (Orecchia, A., et al. (2003). J Cell Sci. 116(Pt 17):3479-3489.).
Inhibition Analysis: A representative lot blocked cell-adhesive epitopes of Fibronectin (Cell Attachment Fragment) (Miller, D. C., et al. (2005). J Biomed Mater Res A. 73(4):476-84.).
Inhibition Analysis: A representative lot blocked RGD-dependent cell adhesion (Tsang, T. M., et al. (2012). J Biol Chem. 287(20):16759-16767.).
Inhibition Analysis: A representative lot inhibited the cell-adhesive epitopes of Fibronectin (Miller, D. C., et al. (2005). J Biomed Mater Res. 73(4):476-484.)
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Fibronectin (Cell Attachment Fragment) in human placenta and liver tissues.
ELISA Analysis: A representative lot detected the conformation of Fibronectin adsorbed onto self-assembled monolayers (SAMs) of alkanethiols over a range of Fibroectin surface densities (Kesolowsky, B. G., et al. (2003). J Biomed Mater Res A. 66(2):247-259.).
Immuncytochemistry Analysis: A representative lot detected Fibronectin (Cell Attachment Fragment) in Extracellular matrix deposited by HUVECs (Orecchia, A., et al. (2003). J Cell Sci. 116(Pt 17):3479-3489.).
Inhibition Analysis: A representative lot blocked cell-adhesive epitopes of Fibronectin (Cell Attachment Fragment) (Miller, D. C., et al. (2005). J Biomed Mater Res A. 73(4):476-84.).
Inhibition Analysis: A representative lot blocked RGD-dependent cell adhesion (Tsang, T. M., et al. (2012). J Biol Chem. 287(20):16759-16767.).
Inhibition Analysis: A representative lot inhibited the cell-adhesive epitopes of Fibronectin (Miller, D. C., et al. (2005). J Biomed Mater Res. 73(4):476-484.)
Quality
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of HepG2 cell lysate.
Target description
~260 kDa observed
Physical form
0.01M Phosphate Buffer containing no preservative, pH 7.1
Format: Purified
Protein G Purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Synthetic peptide with cell attachment activity of fibronectin.
Pierschbacher, M, et al.
Proceedings of the National Academy of Sciences of the USA, 80, 1224-1227 (1983)
Detachment of cells from culture substrate by soluble fibronectin peptides.
Hayman, E G, et al.
The Journal of cell biology, 100, 1948-1954 (1985)
M D Pierschbacher et al.
The Journal of biological chemistry, 257(16), 9593-9597 (1982-08-25)
The complete amino acid sequence of the cell attachment domain of human plasma fibronectin (Pierschbacher, M. D., Hayman, E. G., and Ruoslahti, E. (1981) Cell 26, 259-267) has been determined by automated sequential degradation of a peptic fragment comprising this
Arg-Gly-Asp: a versatile cell recognition signal.
Ruoslahti, E and Pierschbacher, M D
Cell, 44, 517-518 (1986)
Christian Woltersdorf et al.
Matrix biology : journal of the International Society for Matrix Biology, 63, 91-105 (2017-02-14)
Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of β1-integrins
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