CBL412X

Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor^™488 Conjugated

clone TU-20, from mouse, ALEXA FLUOR^™ 488

• Synonym(s): Tubulin beta-4, Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TU-20, monoclonal
• species reactivity: human, mouse, pig, bovine, primate, rat
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_001184110
• UniProt accession no.: Q13509
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TUBB3(10381)

Description

General description

Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.

Specificity

Reactivity with other species has not been determined.

This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.

Immunogen

Epitope: C-terminus

Application

Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor^™488 Conjugated validated for use in IC, IF, WB, IH & IP.

Immunohistochemistry: 5-10 µg/mL on frozen brain tissue.
Although this conjugated antibody will work for Western blotting and Immunoprecipitation, we recommend catalog number CBL412 if these are your primary applications.
Optimal working concentration must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Quality

Routinely tested cortical neurons in Immunocytochemistry.
Immunocytochemical Analysis: 1:250 dilution of this lot detected β-III tubulin in rat cortex cell culture.

Target description

50 kDa

Physical form

Protein G Purified

Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.05% sodium azide.

Storage and Stability

Stable for 6 months at 2-8°C from date of receipt.

Analysis Note

Control
Rat Cortex Cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable