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Key Documents

CB1046

Sigma-Aldrich

Anti-CLDN1 Mouse mAb (1C5-D9)

liquid, clone 1C5-D9, Calbiochem®

Synonym(s):

Anti-Claudin 1

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1C5-D9, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CLDN1(9076)

General description

Purified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.
Recognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.
This Anti-CLDN1 Mouse mAb (1C5-D9) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of CLDN1.

Immunogen

Full-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein

Application





ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3-5 &microg/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells
Positive Control
PRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Guevin C, et al. 2009. Antiviral Res.84, 14.
Sainz, B., Jr, et al. 2009. Virol. J.6, 103.
Stamataki Z, et al. 2009. Blood113 585.
Farquhar MJ, et al. 2008. J. Virol.82 8797.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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