AB1284

Anti-Heme Oxygenase 1 Antibody

Chemicon^®, from rabbit

• Synonym(s): HO-1, HSP32
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: bovine, mouse, rat
• should not react with: canine
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NM_002133.1
• UniProt accession no.: P09601
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Hmox1(513221) ; dog ... Hmox1(442987) ; mouse ... Hmox1(15368) ; rat ... Hmox1(24451)

Description

Specificity

Recognizes Heme oxygenase-1.

Immunogen

A synthetic peptide with the amino acid sequence H-D-L-S-E-A-L-K-E-A-T-K-E-V-H-C-NH2 corresponding to amino acid residues [12-25] + Cys-NH2 of the human HO-1 protein (Yoshida et al., 1988).

Application

Research Category
Metabolism

Research Sub Category
Toxicology & Drug Resistance

This Anti-Heme Oxygenase 1 Antibody is validated for use in IH, WB for the detection of Heme Oxygenase 1.

Western Blot: 1:1,000 (using ECL). A single band of ~32 kD is observed. Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (Catalog Number AG253). Also available are microsomal tissue preparations for control purposes (Rat spleen microsomal preparation, Catalog Number AG257).

Immunohistochemistry: 1:50-1:1,000 on human airway biopsy, human spleen, human kidney and porcine arteries.

Optimal working dilutions must be determined by the end user.

Target description

32 kDa

Physical form

Affinity Purified immunoglobulin. Liquid in PBS containing 0.01M sodium azide.

ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Lung

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable