582231

Tetanus Toxoid, Clostridium tetani

This tetanus toxoid is prepared by formaldehyde inactivation of tetanus toxin. It retains its antigenic character, but is essentially nontoxic as determined by an LD₅₀ in mice.

• Synonym(s): TTx
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 200
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Tetanus Toxoid acts against Clostridium tetani, a gram-positive bacillus that produces a neurotoxin called tetanospasmin. Tetanospasmin prevents the release of an inhibitory neurotransmitter and causes unopposed muscle contractions and spasms. Tetanus toxoid vaccine stimulates the immune system to respond to the vaccine’s antigens. The immune system’s T-lymphocytes (TH2 and B cells) secrete immunoglobulin against the antigen in the vaccine. This immunoglobulin helps to protect against future infections.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 100 mM histidine, 100 mM NaCl, 5% trehalose, pH 7.0.

Reconstitution

Reconstitute in 50 µl of sterile distilled H₂O

Other Notes

Wrenger, S., et al. 2000. J. Biol. Chem.275, 22180.
Habermann E. and Goretzki, K. 1985. In Monoclonal Antibodies Against Bacteria (Macario, A.J.L., and Conway de Macario, E. eds.) Academic Press, New York, p.191.
Kozbor, D. and Roder, J.C. 1981. J. Immunol.127, 1275.
Bizzini, B., et al. 1970. Eur. J. Biochem.17, 100.
Largier, J.F. and Joubert, F.J. 1956. Biochem. Biophys. Acta20, 407.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable