42600-M
Ultrafree® Centrifugal Filter, 0.5 mL Sample Volume
Synonym(s):
DA Centrifugal Filter Unit, DA Centrifugal Filter Unit For DNA extraction, Ultrafree-DA Centrifugal Filter Unit, 0.5 mL
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About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
Recommended Products
material
polypropylene
Quality Level
sterility
non-sterile
manufacturer/tradename
Ultrafree®
parameter
0.5 mL sample volume
50 °C max. temp.
technique(s)
DNA extraction: suitable
L
45 mm
diam.
10.7 mm
filtration area
0.2 cm2
shipped in
ambient
General description
Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).
The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.
Performance
100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29
Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.
- Minimal hands-on time
- 10-minute spin
- Fully-functional DNA
Performance
Typical DNA Recoveries from Agarose Gels
DNA Size (bp) Percent DNA Recoveries
100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29
Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
Application
Fixed-angle microfuge rotor w/maximum 5,000 x g
Legal Information
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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