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401442

Sigma-Aldrich

Goat Anti-Human IgG, γ-Chain Specific Alkaline Phosphatase Conjugate

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

General description

Immunoaffinity purified goat polyclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG γ-chain.
This Goat Anti-Human IgG, γ-Chain Specific Alk-Phos Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG, γ-Chain Specific.

Immunogen

Human

Application

Enzyme Immunoassay (1:5000)

Immunoelectrophoresis (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, pH 8.0.

Other Notes

Monospecific for human IgG γ-chain as determined by immunoelectrophoresis against normal human serum. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pauline Versteegen et al.
Frontiers in immunology, 13, 864674-864674 (2022-06-10)
Immunogenicity of acellular pertussis (aP) vaccines is conventionally assessed by measuring antibody responses but antibody concentrations wane quickly after vaccination. Memory B cells, however, are critical in sustaining long-term protection and therefore may be an important factor when assessing pertussis
Malick M Gibani et al.
Nature medicine, 25(7), 1082-1088 (2019-07-05)
Salmonella Typhi is a human host-restricted pathogen that is responsible for typhoid fever in approximately 10.9 million people annually1. The typhoid toxin is postulated to have a central role in disease pathogenesis, the establishment of chronic infection and human host
Angela M Minassian et al.
Med (New York, N.Y.), 2(6), 701-719 (2021-07-06)
Development of an effective vaccine against the pathogenic blood-stage infection of human malaria has proved challenging, and no candidate vaccine has affected blood-stage parasitemia following controlled human malaria infection (CHMI) with blood-stage Plasmodium falciparum. We undertook a phase I/IIa clinical

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