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Sigma-Aldrich

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ buffer, CAS 9013-72-3, is a large, multi-subunit, O2-carrying, metalloprotein that is used in the production of antibodies.

Synonym(s):

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ Buffer, KLH

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About This Item

CAS Number:
9013-72-3
MDL number:
MFCD00131280
UNSPSC Code:
12352202

Quality Level

100

Assay

≥90% (size exclusion chromatography)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

color

clear blue-gray

shipped in

wet ice

storage temp.

2-8°C

General description

Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. It is isolated from a marine snail species called Megathura crenulate.

Biochem/physiol Actions

Keyhole limpet hemocyanin (KLH) exhibits immunostimulatory properties and stimulates both cell-mediated and humoral responses in animals and humans. It serves as a vaccine adjuvant and facilitates protein conjugation. In addition, KLH acts as a carrier protein to produce antibodies as well as haptens such as peptides and oligosaccharides. It is also used in the treatment of bladder cancer.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM MgSO₄, 5 mM BES buffer, 0.9% NaCl, pH 6.5. Sterile-filtered.

Preparation Note

KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oyindasola Oyelaran et al.
Proteomics. Clinical applications, 4(3), 285-294 (2010-12-08)
Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have
Erik H J G Aarntzen et al.
Cancer immunology, immunotherapy : CII, 61(11), 2003-2011 (2012-04-25)
Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it

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