203189

Biotin-X-NHS, Water-Soluble

• Synonym(s): Biotin-X-NHS, Water-Soluble, Sulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
• Empirical Formula (Hill Notation): C₂₀H₂₉N₄O₉S₂ · Na
• Molecular Weight: 556.59
• UNSPSC Code: 41116133
• NACRES: NA.41

Properties

• Assay: ≥94% (HPLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: off-white to tan
• solubility: DMSO: 13 mg/mL; distilled water: 5.5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Readily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.

Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.

Other Notes

Gretch, D.R., et al. 1987. Anal. Biochem.163, 270.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable