17-127
Ser/Thr Phosphatase Assay Kit 1 (K-R-pT-I-R-R)
Ser/Thr Phosphatase Assay Kit 1used to detect PP2A activity by either dephosphorylation of the phosphopeptide.
Synonym(s):
Phosphatase Assay Kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Recommended Products
Quality Level
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (phosphatase)
NCBI accession no.
UniProt accession no.
detection method
colorimetric
shipped in
wet ice
Gene Information
human ... PPP2R1B(5519)
Application
Ser/Thr Phosphatase Assay Kit 1used to detect PP2A activity by either dephosphorylation of the phosphopeptide.
Used to detect/quantify: Ser/Thr Kinases
Packaging
Kit capacity: 100 assays
Components
Malachite Green Solution A (Cat.# 20-105)
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
NiCl2, 40mM (Cat.# 20-178)
96-well microtiter plate
Malachite Green Additive (Cat.# 20-104)
Phosphate Standard (Cat.# 20-103)
Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)
pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
NiCl2, 40mM (Cat.# 20-178)
96-well microtiter plate
Quality
routinely used to detect PP2A (Catalog # 14-111) activity by either dephosphorylation of the phosphopeptide (K-R-pT-I-R-R) or hydrolysis of pNPP
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Carc. 1A Inhalation - Met. Corr. 1 - Repr. 1B - Skin Sens. 1 - STOT RE 2
Target Organs
Lungs
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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James H Doroshow et al.
Biochemical pharmacology, 83(9), 1195-1207 (2012-02-07)
Flavoprotein-dependent reactive oxygen species (ROS) play a critical role in cytokine-mediated signal transduction in normal tissues and tumor cells. The flavoenzyme inhibitors diphenylene iodonium (DPI) and di-2-thienyliodonium (DTI) have been used to inhibit membrane-bound, flavoprotein-containing NADPH oxidases, including epithelial and
Usha Narayanan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(52), 14349-14357 (2007-12-28)
Fragile X syndrome is a common form of inherited mental retardation and is caused by loss of fragile X mental retardation protein (FMRP), a selective RNA-binding protein that influences the translation of target messages. Here, we identify protein phosphatase 2A
Anna Signorile et al.
FEBS letters, 512(1-3), 91-94 (2002-02-20)
Immunochemical and functional evidence showing the existence in the inner membrane and matrix fraction of mammalian mitochondria of serine/threonine phosphatases acting on cAMP-dependent phosphoproteins is presented. Mg(2+)-dependent Ca(2+)-inhibitable PP2C phosphatase, associated to the inner membrane, dephosphorylates the 18 kDa (NDUFS4
Katsuo Suzuki et al.
International journal of oncology, 23(5), 1263-1268 (2003-10-09)
The beta1 subunit of integrin is serine/threonine phosphorylated in growth arrested human breast cancer MCF-7 cells, while it is not in quiescent normal human breast epithelial (HBE) cells. Using the affinity-purified antibodies PB788-9 against the synthetic oligopeptide that contained phosphothreonines
K S Rutgers et al.
Neuroscience, 190, 37-42 (2011-06-21)
Previously selected amyloid beta recognizing heavy chain antibody fragments (VHH) affinity binders derived from the Camelid heavy chain antibody repertoire were tested for their propensity to cross the blood-brain barrier (BBB) using an established in vitro BBB co-culture system. Of
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