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16-157

Sigma-Aldrich

Protein A Agarose/Salmon Sperm DNA, 2.5 mL

Recombinant protein A covalently bound to agarose beads for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H4 and H3 ChIP assay.

Synonym(s):

ChIP agarose beads, ChIP agaraose A beads, ChIP assays

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32

biological source

Staphylococcus aureus

Quality Level

100

form

liquid

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable

suitability

suitable for immunoprecipitation

shipped in

wet ice

General description

Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Application

Suitable for use in chromatin immunoprecipitation (ChIPs) assays

Quality

Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)

Physical form

10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein Kinase C{zeta} Mediates Cigarette Smoke/Aldehyde- and Lipopolysaccharide-induced Lung Inflammation and Histone Modifications.
Yao H, Hwang JW, Moscat J, Diaz-Meco MT, Leitges M, Kishore N, Li X, Rahman I
The Journal of Biological Chemistry null
Methods for analyzing histone citrullination in chromatin structure and gene regulation.
Pingxin Li,Jing Hu,Yanming Wang
Methods in Molecular Biology null
Mohammad Kamran et al.
Journal of immunology (Baltimore, Md. : 1950), 205(12), 3311-3318 (2020-11-15)
IL-13 plays a critical role in mediating many biological processes responsible for allergic inflammation. Mast cells express Il13 mRNA and produce IL-13 protein in response to antigenic stimulation. Enhancers are essential in promoting gene transcription and are thought to activate
Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo.
Monica E Ferreira, Philippe Prochasson, Kurt D Berndt, Jerry L Workman, Anthony P H Wright
FEBS Journal null
Chromatin immunoprecipitation (ChIP) to assay dynamic histone modification in activated gene expression in human cells.
Lauren J Buro,Shaili Shah,Melissa A Henriksen
Journal of Visualized Experiments null
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