Skip to Content
Merck
All Photos(1)

Key Documents

G005678

HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express RP-Amide

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)
mobile phase: [A] 20 mM ammonium acetate, pH 4.7 with acetic acid; [B] acetonitrile; (92:8, A:B)
flow rate: 0.6 mL/min
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: 500 - 1000 μg/mL in 95:5, buffer: acetonitrile

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Caffeine
    Benzoic acid
    Asp-Phe methyl ester

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service