G004736

HPLC Analysis of Lysozyme on SRT^® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles (Z777051); mobile phase: 50 mM phosphate buffered saline, 200 mM Na₂SO₄, pH 7.0; flow rate: 1 mL/min; column temp.: ambient; detector: UV, 214 nm; injection: 5 μL; sample: 1 mg/mL in 50 mM phosphate buffered saline
• suitability: application for HPLC
• application(s): life science and biopharma

Description

Analysis Note

The positively-charged protein, lyzoyme (pI=11), exhibits excellent peak shape and recovery on SRT SEC-300 columns due to its highly-inert surface. Low protein recovery is usually attributed to 1) hydrophobic interactions with the stationary phase and 2) electrostatic interactions with the silica suface. SRT SEC-300 surface is hydrophilic and densely bonded to silica, therefore providing high mass recovery and good peak shape.

Legal Information

SRT is a registered trademark of Sepax Technologies

Analyte

Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein