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581324-U

Supelco

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

25% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

450 m2/g

surface coverage

3.7 μmol/m2

impurities

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Features and Benefits

  • Excellent retention
  • Symmetric peak shape
  • High reproducibility
  • Complete LC-MS compatibility

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Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
282406-06
282406-05
282406-04
282406-03
282406-02

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Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
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Articles

Ion Pairing for Analysis of Phosphonate Compounds

Phosphonates are a class of compounds commonly used for therapeutic applications of antiviral drugs. These compounds can be difficult to analyze due to their highly polar nature and lack of UV active chromophores.

Protocols

Analysis of Difficult Polar Compounds using Sigma-Aldrich Ion Pair Reagents and Supelco Ascentis HPLC Columns

Because reversed-phase HPLC is primarily dependent on hydrophobic interactions between the stationary phase and analyte, ion pairing is occasionally necessary to obtain sufficient retention of polar, ionizable compounds.

HPLC Analysis of Phenylacetic Acid on Ascentis® C18

Separation of Phenylacetic acid

HPLC Analysis of Penicillin G and Phenoxymethylpenicillinic Acid on Ascentis® C18

Separation of Phenoxymethylpenicillinic acid

HPLC Analysis of Prednisolone (Secondary Pharma Standard) on Ascentis® C18

Separation of Prednisolone, pharmaceutical secondary standard; traceable to USP, PhEur and BP

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Chromatograms

application for HPLCapplication for HPLC

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