description
Silica Column Regeneration Solution
packaging
pkg of 200 mL
compatibility
Restoring Column Performance
storage temp.
room temp
General description
Silica Column Regeneration Solution contains a mixture of acetic acid, dimethoxypropane, and methylene chloride.
This solution effectively regenerates a silica column that has come into contact with very strongly polar solvents, such as water or alcohols. Simply flush the column with regeneration solution for 10 minutes, then reequilibrate with mobile phase for 10 minutes. Column performance usually is restored to that obtained before exposure to the polar solvent.
Application
It was used to flush and regenerate the column used for HPLC analysis of tocopherols and tocotrienols.
Analyte
Description
Acetic acid
Dichloromethane
2,2-Dimethoxypropane
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Simple solutions to problems encountered in quantitative analysis of tocopherols and tocotrienols using silica columns.
A K Hewavitharana
Analytical biochemistry, 313(2), 342-344 (2003-02-28)
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