ZRB1509

Anti-V5 Tag Antibody, clone 1K20-L2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Anti-Adiponectin Antibody, clone 1D19 ZooMAb^® Rabbit Monoclonal, Non-structural protein V tag
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1K20-L2, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 23.94 kDa
• purified by: using Protein A
• species reactivity: all
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: N/A
• UniProt accession no.: P11207
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1K20-L2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects proteins with V5 tag. It targets an epitope within 14 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the internal region of V5 protein from the Parainfluenza virus 5.

Application

Quality Control Testing

Evaluated by Western Blotting with His/V5-tagged recombinant Tau-441 protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His/V5-tagged recombinant Human Tau-441 protein.

Tested Applications

ELISA Analysis: A serial of dilutions from a representative lot detected V5-tagged recombinant Human Tau-441 protein.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HEK293 cells transfected with a V5 expression plasmid.

Peptide Inhibition Assay:: Target band detection in V5-tagged recombinant Human Tau-441 protein was prevented by preblocking of a representative lot with V5 tag peptide, but not the corresponding non-V5 tag control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Non-structural protein V (UniProt: P11207; also known as V5 tag) is encoded by the P/V gene in Parainfluenza virus 5. Epitope tags are genetically engineered short protein sequences that are serve as universal epitopes and can be easily detected with antibodies without affecting the native structure or function of proteins. The V5 tag is a sequence of 14 amino acids (GKPIPNPLLGLDST) derived from the internal region of non-structural protein V of parainfluenza virus 5. It can be used to detect expression of recombinant proteins in bacteria, insects, and mammalian cell systems. It is useful in detecting recombinant proteins that contain the V5 epitope. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable