T9568

Trizma^® Pre-set crystals

BioPerformance Certified, pH 8.9, average M_w 125.6

• Synonym(s): Tris crystals
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Quality Level: 200
• form: crystalline powder
• mol wt: average M_w 125.6
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 8.90 (25 °C, 0.1 M)(lit.); 8.9
• useful pH range: 7.0-9.0
• solubility: water: 0.66 g/mL, clear, colorless
• application(s): diagnostic assay manufacturing

Description

Application

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves