Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

T8823

Sigma-Aldrich

Monoclonal Anti-T7 tag antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

Synonym(s):

T7 Tag antibody

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD02689159
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T7 Tag

form

buffered aqueous solution

concentration

~2 mg/mL

technique(s)

western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.
The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein.

Immunogen

synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH

Application

Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.

Physical form

Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

Antibody is suitable for ELISA and immunoblotting.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Prachi Singh et al.
Medical oncology (Northwood, London, England), 34(8), 145-145 (2017-07-22)
DNA methyltransferases make use of alternative splicing mechanism to generate various splice variants, but their role(s) in modulating DNA methylation patterns in the cells is unclear. Notably, DNMT3B alone contains nearly 40 different splice variants. In this study, we have
Design of high-throughput methods of protein production for structural biology
Stevens RC
Structure, 8(9), R177-R185 (2000)
Protein 4.1 R influences myogenin protein stability and skeletal muscle differentiation
Huang SC, et al.
Test, 291(49), 25591-25607 (2016)
Tumor necrosis factor: methods and protocols, 98, 12-12 (2004)
ESSENTIALS OF MEDICINAL CHEMISTRY (2013)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service