SRP6267

C-reactive/CRP human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): C-reactive protein, CRP, PTX1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: calculated mol wt 24 kDa; observed mol wt 26 kDa (DTT-reduced. Phe17 is the predicted N-terminus.)
• packaging: pkg of 100 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P02741
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CRP(1401)

Description

General description

C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat, respectively.

Biochem/physiol Actions

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable