SRP6119

DHFR from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): DYR, Dihydrofolate reductase, N/A
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: 23.8 kDa (207 aa, 1-187 aa + NT His-Tag)
• packaging: pkg of 100 μg
• NCBI accession no.: P00375
• UniProt accession no.: P00375
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: mouse ... DHFR(13361)

Description

General description

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. The gene encoding this protein is localized on mouse chromosome 13.

Biochem/physiol Actions

DHFR catalyzes the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Physical form

1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable