SRE0068

SSC Buffer, 20X Concentrate

• Synonym(s): Saline Sodium Citrate Buffer
• UNSPSC Code: 41105600
• NACRES: NA.25

Properties

• form: liquid
• Quality Level: 500
• application(s): diagnostic assay manufacturing
• storage temp.: 2-8°C

Description

Application

SSC Buffer, 20X Concentrate, is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X. It is also for use in preparing nucleic acids for hybridization.

Physical form

This SSC Buffer, 20X Concentrate, contains 0.3 M sodium citrate in 3 M NaCl. Preparation of 1X SSC working buffer: Dilute the 20X concentrated buffer 20-fold with ultrapure water (= 18 MΩ x cm resistivity at 25 °C).

Notes: If precipitation is present in the 20X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution. It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable