SAB4200816

Anti-Calcineurin (α-Subunit) antibody, Mouse monoclonal

clone CN-A1, purified from hybridoma cell culture

• Synonym(s): CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: CN-A1, monoclonal
• species reactivity: bovine, human, rat
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1.25-2.5 μg/mL using mouse or rat brain tissue extract.
• isotype: IgG1
• UniProt accession no.: Q08209
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... PPP3CA(286852) ; human ... CALNA(5530) ; rat ... Caln1(363909)

Description

General description

Calcineurin (a-subunit) (CnA), also known as Serine/threonine-protein phosphatase 2B catalytic subunit alpha (PP3CA), Calmodulin-dependent calcineurin A subunit alpha (CALN/CALNA) or CAM-PRP catalytic subunit, is a major soluble calmodulin-binding protein in the brain. Calcineurin is a heterodimer metalloenzyme, Ca2+/Calmodulin dependent serine/threonine protein phosphatase 3 (PPP3), with a relatively narrow substrate specificity4-6,8 It is composed of two subunits, a catalytic calmodulin-binding a subunit (61 kDa, calcineurin A [CnA]) and regulatory calcium-binding ß subunit (18 kDa, calcineurin B [CnB]).6,7 Both subunits are highly conserved from yeast to mammals.8

Specificity

Monoclonal Anti-Calcineurin specifically recognizes an epitope located on the a-subunit of calcineurin from human, mouse, monkey, porcine1, rat2 and bovine13 origin. It does not cross-react with calcineurin ß-subunit.

Immunogen

Purified bovine brain calcineurin.

Application

The antibody may be used in various immunochemical techniques including Immunoblot1,2 (~61 kDa), ELISA and Immunohistochemistry1,3.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable