S5265

Streptolysin O from Streptococcus pyogenes

lyophilized powder, Protein ~3 % by Lowry, 25,000-50,000 U/vial

• CAS Number: 98072-47-0
• EC Number: 308-500-3
• MDL number: MFCD00132389
• UNSPSC Code: 51101500
• NACRES: NA.85

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: 25,000-50,000 U/vial
• mol wt: native 69 kDa
• composition: Protein, ~3% Lowry
• storage condition: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• color: white
• solubility: H₂O: soluble 1 mg/mL
• antibiotic activity spectrum: neoplastics
• Mode of action: cell membrane | interferes
• storage temp.: 2-8°C
• Gene Information: Streptococcus pyogenes MGAS8232 ... slo(993428)

Description

Application

Streptolysin O is a toxin secreted by Streptococcus pyogenes and is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy.

Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs.

Permeabilizes membranes to permit cellular uptake of large or charged molecules.

Biochem/physiol Actions

Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass. It is thiol-activated. It is inhibited by allicin, an active component of garlic.

Thiol-activated toxin that permeabilizes animal cell membranes. The protein binds as a monomer to membrane cholesterol and subsequently polymerizes into large arc- and ring-shaped structures surrounding pores of >12 nm.

Packaging

25KU

Unit Definition

One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min.

Physical form

Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place

Safety Information

• Pictograms: GHS05,GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H300 + H310 + H330 - H315 - H318 - H411
• Precautionary Statements: P260 - P262 - P273 - P280 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391 - P403 + P233
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves