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Key Documents

RAB0053

Sigma-Aldrich

Mouse MIP-3 β / CCL19 ELISA Kit

for serum, plasma and cell culture supernatant

Synonym(s):

C-C motif chemokine 19, CCL19, MIP-3 beta

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 4.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Ccl19(24047)

General description

C-C motif chemokine ligand 19 (CCL19), also known as macrophage inflammatory protein (MIP)-3β, is encoded by the gene mapped to human chromosome 9. The encoded protein is specifically expressed in lymphoid tissues.
The Mouse MIP-3-β (Macrophage Inflammatory Protein 3 β) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-3- β in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse MIP-3 β

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

C-C motif chemokine ligand 19 (CCL19) acts as a chemoattractant for T and B lymphocytes, dendritic cells, macrophage progenitor cells and natural killer cells. Therefore, this protein might play an essential role in migration of T cells in the thymus and transfer of T and B cells to secondary lymphoid organs. Overexpression of MIP-3 β (macrophage inflammatory protein-3β)/ C-C motif chemokine ligand 19 (CCL19) leads to pulmonary sarcoidosis.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
1014I0448
1124H0448
0119I0448
1008H0448
SAMPLE

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Expression of macrophage inflammatory protein-3 beta/CCL19 in pulmonary sarcoidosis.
Gibejova A
American Journal of Respiratory and Critical Care Medicine, 167(12), 1695-1703 (2003)
CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells.
Marsland BJ
Immunity, 22(4), 493-505 (2005)
The CCR7 ligand elc (CCL19) is transcytosed in high endothelial venules and mediates T cell recruitment.
Baekkevold ES
The Journal of Experimental Medicine, 193(9), 1105-1112 (2001)

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