R7755
RPMI-1640 Medium
AutoMod™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture
Pharma Manufacturing
Synonym(s):
Roswell Park Memorial Institute 1640 medium
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About This Item
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Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
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General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Other Notes
Modified for autoclaving
Without L-glutamine and sodium bicarbonate.
Without L-glutamine and sodium bicarbonate.
Quantity
Formulated to contain 10.3 grams of powder per liter of medium.
Reconstitution
Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.
Legal Information
Auto-Mod is a trademark of Sigma-Aldrich Co. LLC
also commonly purchased with this product
Product No.
Description
Pricing
supplement
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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