R5280

Anti-phospho-RanGAP1 (pSer⁴²⁸) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Ran GTPase activating protein-1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using HeLa cells; western blot: 1.5-3.0 μg/mL using HEK-293T cell lysate expressing human RanGAP1
• UniProt accession no.: P46060
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer428)
• Gene Information: human ... RANGAP1(5905)

Description

General description

The gene for Ran GTPase activating protein 1 (RANGAP1) is located on the human chromosome 22q13.2.

Specificity

Anti-phospho-RanGAP1 (pSer428) specifically recognizes human phospho-RanGAP1 (pSer428) (not yet tested in other species).

Application

Anti-phospho-RanGAP1 (pSer428) antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

However, the SUMO-1 modification induces the interaction of RanGAP1 with the interphase nuclear pore complex (NPC) through binding to the nucleoporin Ras-related nuclear protein 1 binding protein 2 (RANBP2) and to Ubc9. RanGAP1 is phosphorylated on residues Thr409, Ser442 and Ser428. Phosphorylated RanGAP1 may aid the translocation of specific SUMO target proteins to RanBP2′s catalytic domain.

Ran GTPase Activating Protein 1 (RanGAP1) is a key regulator of Ran activity, by specifically inducing its GTPase activity. RanGAP1 is conjugated to the small ubiquitin-related modifier protein (SUMO-1). The activity of RanGAP1 is not substantially altered by SUMO-1 modification. Phosphorylation ensues before the breakdown of the nuclear envelope and is sustained during the entire mitosis process.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves