N218
Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse
clone NOS-3F7-B11-B5, ascites fluid
Synonym(s):
Anti-IHPS1, Anti-N-NOS, Anti-NC-NOS, Anti-NOS, Anti-bNOS, Anti-nNOS
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About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NOS-3F7-B11-B5, monoclonal
species reactivity
mouse, bovine, rat
technique(s)
immunohistochemistry (frozen sections): 1:100
western blot: 1:50-1:500
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Nos1(18125)
rat ... Nos1(24598)
Specificity
The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS).
Immunogen
bovine cerebellum NOS.
Biochem/physiol Actions
Neuronal nitric acid synthase (Nos1) in involved in the production of nitric oxide. Mutations in the gene encoding it increases the susceptibility to behavioral dysfunction after an exposure to alcohol in mice.
Physical form
Ascites fluid containing ≤0.1% sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Liliana Rytel
International journal of molecular sciences, 19(10) (2018-10-03)
Bisphenol A (BPA), a substance commonly used in the manufacture of plastics, shows multidirectional negative effects on humans and animals. Due to similarities to estrogens, BPA initially leads to disorders in the reproductive system. On the other hand, it is
Bahri Karacay et al.
Alcoholism, clinical and experimental research, 39(2), 221-231 (2015-02-11)
Prenatal alcohol exposure can kill developing neurons, leading to microencephaly and mental retardation. However, not all fetuses are equally vulnerable to alcohol's neurotoxic effects. While some fetuses are severely affected and are ultimately diagnosed with fetal alcohol syndrome (FAS), others
Dimka Hinova-Palova et al.
Journal of molecular histology, 39(4), 447-457 (2008-08-08)
Nitric oxide is a unique neurotransmitter, which participates in many physiological and pathological processes in the organism. Nevertheless there are little data about the neuronal Nitric Oxide Synthase immunoreactive (nNOS-ir) neurons and fibers in the dorsal claustrum (DC) of a
A Rengasamy et al.
The American journal of physiology, 267(6 Pt 1), L704-L711 (1994-12-01)
We addressed the controversial role of nitric oxide (NO) in bronchial function by an immunohistochemical study of the localization of NO synthase (NOS) and its effector protein, soluble guanylate cyclase, in rat bronchus. For this study, a monoclonal antibody to
C Xue et al.
The American journal of physiology, 267(6 Pt 1), L667-L678 (1994-12-01)
Expression and localization of nitric oxide synthase (NOS) in the lungs of chronically hypoxic and normoxic rats were studied using both immunohistochemistry and NADPH diaphorase (NADPH-d) staining techniques. In the normoxic and in the hypoxic rat, NOS was detected by
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