MAK142
Fluorometric Intracellular Ros Kit
sufficient for 200 fluorometric tests (Deep red)
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About This Item
Recommended Products
usage
sufficient for 200 fluorometric tests (Deep red)
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
Related Categories
General description
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.
Application
- Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells.: This article uses fluorometric methods to explore the anti-inflammatory and antioxidative properties of diethyl blechnic, illustrating the role of ROS assays in elucidating the molecular mechanisms underlying the therapeutic effects of natural compounds (Yang S et al., 2019).
Suitability
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.
Principle
The Fluorometric Intracellular ROS Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells after 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and with detection at λex = 650/λem = 675 nm.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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Inhibition of autophagy enhances the selective anti-cancer activity of tigecycline to overcome drug resistance in the treatment of chronic myeloid leukemia.
Lu Z, et al.
Journal of Experimental & Clinical Cancer Research, 36(1), 43-43 (2017)
Reactive oxygen species: Re-evaluation of generation, monitoring and role in stress-signaling in phototrophic organisms.
Schmitt F J, et al.
Biochimica et Biophysica Acta - Bioenergetics, 1837(6), 835-848 (2014)
Jian-An Pan et al.
Redox biology, 46, 102120-102120 (2021-09-04)
The dose-dependent toxicity to cardiomyocytes has been well recognized as a central characteristic of doxorubicin (DOX)-induced cardiotoxicity (DIC), however, the pathogenesis of DIC in the cardiac microenvironment remains elusive. Irisin is a new hormone-like myokine released into the circulation in
Tereza C Cardoso et al.
Journal of neurovirology, 22(6), 725-735 (2016-06-18)
Oncolytic viruses have the ability to infect tumor cells and leave healthy cells intact. In this study, bovine herpesvirus 1 (BHV1; Los Angeles, Cooper, and SV56/90 strains) and bovine herpesvirus 5 (BHV5; SV507/99 and GU9457818 strains) were used to infect
The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults.
Nita M and Andrzej G
Oxidative Medicine and Cellular Longevity, 2016 (2016)
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