MAK114
Glyoxalase I Activity Assay Kit
sufficient for 100 tests (UV)
Synonym(s):
Aldoketomutase Activity Assay, GLO1 Activity Assay, Ketone-Aldehyde Mutase, Activity Assa, Lactoylglutathione Lyase Activity Assay, Methylglyoxalase Activity Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 tests (UV)
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
human ... GLO1(2739)
mouse ... GLO1(109801)
rat ... GLO1(294320)
General description
Glyoxalase I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methyglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans, and cancer.
Application
Glyoxalase I Assay Kit has been used to estimate the glyoxalase I activity in plasma samples.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of Glyoxalase I activity in enzyme preparations and biological samples. Suitable for testing the effects of compounds on Glyoxalase I activity.
Principle
The Glyoxalase I Activity Assay kit provides a simple and direct procedure for measuring GLO-1 activity in a variety of samples such as enzyme preparations or biological samples. In this assay, the GLO-1-mediated production of S-lactoylglutathione is measured by monitoring the change in absorbance at 240 nm. One unit of Glyoxalase I is the amount of enzyme that will convert 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.6 and 25 °C.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
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Journal of applied physiology (Bethesda, Md. : 1985), 124(2), 330-340 (2017-09-25)
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In plants, lineage-specific metabolites can be created by activities derived from the catalytic promiscuity of ancestral proteins, although examples of recruiting detoxification systems to biosynthetic pathways are scarce. The ubiquitous glyoxalase (GLX) system scavenges the cytotoxic methylglyoxal, in which GLXI
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Methylglyoxal was shown to impair adipose tissue capillarization and insulin sensitivity in obese models. We hypothesized that glyoxalase-1 (GLO-1) activity could be diminished in the adipose tissue of type 2 diabetic obese patients. Moreover, we assessed whether such activity could
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