M7292

M16 Medium

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

• Synonym(s): Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | embryo: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: 2.101 g/L; phenol red: 0.0106 g/L; sodium pyruvate: 0.0363 g/L; glucose: 1.0 g/L (Dextro)
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Quality

Presence of particulates in solution will not affect product′s performance.

Reconstitution

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable