M3942

DMEM - high glucose

MegaCell^™, with sodium pyruvate, without ʟ-glutamine, sterile liquid, suitable for cell cutlure

• Synonym(s): DMEM, DMEM Supplement
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: MegaCell^™ Dulbecco′s Modified Eagle′s Medium, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: no; glucose: 4500 mg/L (Dextro); sodium pyruvate: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

MegaCell^™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.

Application

MegaCell^™ Dulbecco′s Modified Eagle′s Medium has been used:

• as a component of the M10 medium to culture CD133⁺ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
• as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
• to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies

Reconstitution

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Legal Information

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.

MegaCell is a trademark of Promega Corp.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable